Fluorescence microscopy

Details Fluorescence microscopy Fluorescence microscopy

1986-10-02

1988-12-13

Fields, Carolyn E.

Radiant energy

Luminophor irradiation

2504612, G01N 2131

Patent

active

047913100

ABSTRACT:
A multi-chronal fluorescence imaging technique for spatial differentiation and correlation of a plurality of separate sample components tagged with site specific dyes is described. The dyes have decay times that are widely separated and vary by approximately a factor of ten from one dye to the next. These are added to the sample and are excited with a short pulse of ultraviolet light. Between the sample and a detector is placed an adjustable shutter or gate which is opened and closed at predetermined intervals so that the detector can see the approximate maximum intensity output of each dye in a shown ordered sequence without significant interference from the other dyes.

REFERENCES:
patent: 4006360 (1977-02-01), Mueller
patent: 4058732 (1977-11-01), Wieder
patent: 4236071 (1980-11-01), Chimenti
patent: 4573796 (1986-03-01), Martin et al.
patent: 4626693 (1986-12-01), Hirschfeld

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