Fluorescence microscope and observation method using the same

Details Fluorescence microscope and observation method using the same Fluorescence microscope and observation method using the same

2007-11-20

2007-11-20

Epps, Georgia (Department: 2878)

Radiant energy

Luminophor irradiation

C356S417000, C356S213000, C356S135000, C250S462100

Reexamination Certificate

active

10975707

ABSTRACT:
The present invention relates, in general, to a fluorescence microscope and method of observing samples using the microscope and, more particularly, to a fluorescence microscope and method of observing samples using the microscope, which can reduce optical noise and obtain images with higher sensitivity, thus obtaining precise information about the density, quantity, location, etc. of a fluorophore, and which can simultaneously process separate images even when a plurality of fluorophores having different excitation and fluorescent wavelength ranges is distributed, thus easily obtaining information about the fluorophores. The fluorescence microscope of the present invention includes an objective lens, and first and third medium units. The first medium unit has a refractive index of n1to accommodate one or more micro-objects including fluorophores and provide a path of excitation light to excite the fluorophores. The third medium unit has a refractive index of n3, and is placed between the first medium unit and the objective lens to totally reflect the excitation light incident through the first medium unit at an interface of the third medium unit coming into contact with the first medium unit. The refractive indices of the third and first medium units satisfy a relationship of n1>n3.

REFERENCES:
patent: 4621911 (1986-11-01), Lanni et al.
patent: 5633724 (1997-05-01), King et al.
patent: 6201989 (2001-03-01), Whitehead et al.
patent: 6255083 (2001-07-01), Williams
patent: 2002/0097489 (2002-07-01), Kawano et al.
patent: 2003/0157538 (2003-08-01), Krull et al.
Abramowitz, et al. “Refraction of Light”. Molecular Expressions: Science, Optics, and You: Light and Color—Refraction of Light. Last Modified Aug. 1, 2003. http://www.olympusmicro.com/primer/lightandcolor/refraction.html.
Applied Precision, LLC., “arrayWoRx®e4-Color Biochip Reader” brochure, 2004.

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