Fluorescence method for enzyme analysis which couples aromatic a

Details Fluorescence method for enzyme analysis which couples aromatic a Fluorescence method for enzyme analysis which couples aromatic a

1978-11-13

1980-10-21

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

G01N 3114

Patent

active

042295289

ABSTRACT:
Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes.

REFERENCES:
patent: 3591459 (1971-07-01), Haschen et al.
patent: 3862011 (1975-01-01), Smith

Affiliated with

Also associated with

Rating

Fluorescence method for enzyme analysis which couples aromatic a does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescence method for enzyme analysis which couples aromatic a, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescence method for enzyme analysis which couples aromatic a will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2023579