Fluorescence-measuring system

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

Reexamination Certificate

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C250S458100

Reexamination Certificate

active

06985225

ABSTRACT:
The invention relates to a fluorescence-measuring system as can be employed for high-throughput screening in drug development. The arrangement for fluorescence excitation contains a two-dimensionally extended sample-receiving device and at least two illumination sources for exciting the fluorescence of the samples. The illumination sources are extended linearly and arranged in such a way that the illuminated area of the sample-receiving device is homogeneously illuminated directly or via deflecting mirrors at an opening angle of ≦30°. A detector system for the fluorescence light from the sample-receiving device is arranged on either side of the sample-receiving device in such a way that it detects fluorescence emission from the area of measurement at an angle outside the range of reflection of the excitation light of the illumination sources at the illuminated area of the sample-receiving device, preferably at an angle in the range from 80° to 100°, particularly preferably about 90°, to the extended plane of the area of the sample-receiving device.

REFERENCES:
patent: 5355215 (1994-10-01), Schroeder et al.
patent: 5854684 (1998-12-01), Stabile et al.
patent: 5933232 (1999-08-01), Atzler et al.
patent: 6057163 (2000-05-01), McMillan
patent: 6542241 (2003-04-01), Thorwirth et al.
patent: 6856390 (2005-02-01), Nordman et al.
patent: 2003/0010930 (2003-01-01), Thorwirth
patent: 19914279 (2000-07-01), None
patent: 0751393 (1997-02-01), None
patent: 2808888 (2001-11-01), None
patent: 9842442 (1998-01-01), None
Schroeder, K. et al., FLIPR: A New Instrument for Accurate, High Troughput Optical Screening: J. of. Biomolecular Screening, 1, 75-80, (1996).

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