Fluorescence ligand binding assay using charge-matched dye and s

Details Fluorescence ligand binding assay using charge-matched dye and s Fluorescence ligand binding assay using charge-matched dye and s

1984-07-09

1989-03-28

Warden, Robert J.

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

436501, 436536, 436537, 436800, 436826, 436829, 435810, G01N 33533

Patent

active

048164194

ABSTRACT:
Fluorescence ligand binding assay of a sample containing an unknown amount of ligand is performed by making direct intensity measurements. In an immunoassay the sample is in a solution containing dye labeled analyte and an antibody specific to the analyte. Surfactant, added to the solution in an amount sufficient to form micelles, provides markedly different fluorescent intensity from bound and unbound labeled analyte if the surfactant ions and dye ions have the same charge polarity and the analyte moeity has the opposite charge polarity.

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