Fluorescence lifetime measurement apparatus

Details Fluorescence lifetime measurement apparatus Fluorescence lifetime measurement apparatus

2007-05-08

2007-05-08

Lauchman, Layla G. (Department: 2877)

Optics: measuring and testing

By dispersed light spectroscopy

With sample excitation

C356S318000

Reexamination Certificate

active

10912784

ABSTRACT:
A fluorescence lifetime measurement apparatus includes a first controller controlling a total gate length and a second controller controlling a start gate length of a plurality of gate lengths into which the total gate length is divided. The start gate length is a time window whose start time is when measurement of the number of fluorescence photons of a specimen starts. The apparatus also includes a measurer measuring the number of fluorescence photons for each of the gate lengths; a processor calculating a fluorescence lifetime the specimen based on the start gate length and the number of fluorescence photons; and a third controller causing, when the fluorescence lifetime does not satisfy a predetermined condition, the first and second controllers to change the start gate length and the total gate length.

REFERENCES:
patent: 5485530 (1996-01-01), Lakowicz et al.
patent: 6809816 (2004-10-01), Sharma
patent: 2005/0072936 (2005-04-01), Kotani
patent: 2382648 (2003-06-01), None
patent: 2002-107300 (2002-04-01), None
H.C. Gerritsen et al; Fluorescence Lifetime Imaging in Scanning Microscopes: Acquiisition Speed, Photon Economy and Lifetime Resolution; 2002 The Royal Microscopical Society; vol. 206; Jun. 2002; pp. 218-223.

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