Fluorescence intensity multiple distributions analysis:...

Details Fluorescence intensity multiple distributions analysis:... Fluorescence intensity multiple distributions analysis:...

2005-11-15

2005-11-15

Porta, David (Department: 2878)

Radiant energy

Luminophor irradiation

Methods

C702S028000

Reexamination Certificate

active

06965113

ABSTRACT:
A method for distinguishing samples having flourescent particles includes (a) monitoring intensity fluctuations of fluorescence emitted by the particles in at least one measurement volume by detecting sequences of photon counts by at least one photon detector, (b) determining, from the sequences of photon counts, intermediate statistical data involving at least two probability functions of the number of photon counts detected in different sets of counting time intervals, and (c) determining from the intermediate statistical data a distribution of particles as a function of at least two arguments, wherein one argument is a specific brightness of the particles, or a measure thereof, and another argument is a diffusion coefficient of the particles, or a measure thereof.

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Hong Oian et al., “On The Analysis of High Order Moments of Fluorescence Fluctuations”, vol. 57, pp. 375-380, Feb. 1990, Biophys. J.

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