Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Reexamination Certificate
2005-09-06
2005-09-06
Ceperley, Mary E. (Department: 1641)
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
C436S527000, C436S546000, C436S800000, C436S805000, C436S829000
Reexamination Certificate
active
06939721
ABSTRACT:
The invention includes a composition of matter and method that utilizes energy transfer between one or more donor and acceptor molecules. The composition of matter includes an encapsulation vesicle having a matrix, a surface coating of an organo-metallic complex and a transparent protection layer. The transparent protection layer is capable of modification by addition of biomolecules to the surface in order to bind other molecules. The proximity of the bound biomolecules to the protective layer allows for energy transfer from a donor molecule internal to the protection layer to an acceptor molecule outside the protection layer. The protection layer acts to diminish the effects of collisional quenching on the donor molecules caused by ubiquitous small molecules such as molecular oxygen. The application also teaches a method of making and applying the complexes to immunoassays.
REFERENCES:
patent: 4372745 (1983-02-01), Mandle et al.
patent: 5521289 (1996-05-01), Hainfeld et al.
patent: 5631169 (1997-05-01), Lakowicz et al.
patent: 6528165 (2003-03-01), Chandler
patent: 00/32044 (2000-06-01), None
Jiyan Chen and Paul R. Selvin, “Thiol-Reactive Luminescent Chelates of Terbium and Europium”, Bioconjugate Chem., 1999, 10, 311-315.
Gerard Mathis, “Rare Earth Cryptates and Homogenous Fluorimmunoassays with Human Sera”, Clin. Chem., 39(9), 1993, 1953-1959.
Gerard Mathis, “Probing Molecular Interactions with Homogenous Techniques Based on Rare Earth Cryptates and Fluorescenc Energy Transfer”, Clin. Chem. 41(9), 1995, 1391-1397.
P. Aich, et al., “M-DNA: A Complex Between Divalent Metal Ions and DNA which Behaves as a Molecular Wire”, J. Mol. Biol 1999, 294, 477-485.
Y. Zhou, et al., “Preparation of Hyperbranched Polymer Films Grafted on Self-Assembled Monolayers”, J. Am. Chem. Soc. 118 (1996) 3773-3774.
Ilkka Hemmila, t al., “Time-resolved Fluorometry: An Overview of the Labels and Core Technologies for Drug Screening Applications”, DDT vol. 2, No. 9, Sep. 1997, 373-381.
Edward M. Kober, et al., “Synthetic Control of Excited States. Nonchromophoric Ligand Variations in Polypyridyl Complexes of Osmium (II)”. Inorg. Chem. 1985, 24, 2755-2763.
Mauze Ganapati R.
Yang Dan-Hui
Agilent Technologie,s Inc.
Ceperley Mary E.
Joyce Timothy H.
LandOfFree
Fluorescence immunoassays using organo-metallic complexes... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fluorescence immunoassays using organo-metallic complexes..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescence immunoassays using organo-metallic complexes... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3369509