Fluorescence immunoassay using water insoluble dyes

Details Fluorescence immunoassay using water insoluble dyes Fluorescence immunoassay using water insoluble dyes

1987-10-19

1990-12-18

Kepplinger, Esther L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436528, 436546, 436800, 436808, 436829, G01N 33543

Patent

active

049786253

ABSTRACT:
In a method for fluorescence immunoassay of an analyte, an antianalyte attached to the surface of a solid support is contacted with the analyte and a tracer which includes a substantially water insoluble fluorescent dye occluded in the nonaqueous portion of a sac. After binding reactions involving the antianalyte, analyte and tracer, the solid support is separated, excitation light is applied and fluorescence from dye in intact sacs bound to the solid support is measured and compared to fluorescence measured when known quantities of analyte are assayed. The invention includes a kit of materials useful in performing an immunoassay in accordance with the method of the invention.

REFERENCES:
patent: 4058732 (1977-11-01), Wieder
patent: 4235792 (1980-11-01), Hsia et al.
patent: 4235871 (1980-11-01), Papahadjopoulos et al.
patent: 4283382 (1981-08-01), Frank et al.
patent: 4342826 (1982-08-01), Cole
patent: 4372745 (1983-02-01), Mandle et al.
patent: 4373932 (1983-02-01), Gribnau et al.
patent: 4374120 (1983-02-01), Soimi et al.
patent: 4605630 (1986-08-01), Kung et al.
patent: 4656129 (1987-04-01), Wagner
patent: 4695554 (1987-09-01), O'Connell et al.
patent: 4703017 (1987-10-01), Campbell et al.
patent: 4717655 (1988-01-01), Fulioyler
Truneh, A., et al., Journ. Immunol. Methods 100:59-71 (1987).
Raue, R. et al., Heterocycles 21 No. 1: 167-190 (1984).
Dakubu, S. et al., Clin Biochem Anal., 14:71-101 (1984).
Hemmila, I. et al, Anal. Biochem., 137:335-343 (1984).

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