Fluorescence immunoassay method utilizing pseudo-antigens combin

Details Fluorescence immunoassay method utilizing pseudo-antigens combin Fluorescence immunoassay method utilizing pseudo-antigens combin

1992-02-03

1993-07-20

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

436546, 436816, G01N 33542

Patent

active

052293025

ABSTRACT:
A fluorescence immunoassay method is described in which the presence or absence of antigen is measured within one minute by measuring a variation in fluorescence emitted from an antibody. The method comprises reaction between a pseudo-antigen obtained by chemical combination of an antigen and a fluorescent quencher and an antibody by which fluorescence to be emitted from the antibody is quenched by the action of the quencher combined. When an analyte is added to a solution of the combination, the pseudo-antigen is replaced by an antigen if the analyte contains the antigen, so that the fluorescence increases in intensity. This intensity is measured and compared with the initially measured intensity to detect the presence of the antigen. The method does not make use of the action of antigen on antibody with respect to the fluorescence of the antibody and is applicable to all antigens.

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