Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation
Patent
1997-08-07
1999-08-24
Evans, F. L.
Optics: measuring and testing
By dispersed light spectroscopy
With sample excitation
356417, 2504581, G01N 2164
Patent
active
059431296
ABSTRACT:
A fluorescence imaging system for epi-illumination wherein the usual dichroic beamsplitter is replaced by a polarizing beamsplitter (PBS). The sample is excited with light that is linearly polarized to a significant degree, and fluorescent emission light is collected in the orthogonal linear polarization state. Excitation light that is scattered or reflected by the sample is rejected by the PBS, while the desired emission light is captured for imaging by a detector. By eliminating the usual dichroic beamsplitter member, the imaging system removes the barriers to multi-spectral imaging that such members conventionally impose. A wavelength-selective birefringent network may also be interposed between the beamsplitter and the sample for converting the polarization of either the excitation or the emission light to the orthogonal state without defeating this desirable rejection property, thus permitting measurement of the sample emissions in either or both linear polarization states for assessing fluorescence polarization.
REFERENCES:
patent: 3705755 (1972-12-01), Baer
patent: 3785714 (1974-01-01), Hock et al.
patent: 3860813 (1975-01-01), Herzog et al.
patent: 4122348 (1978-10-01), Bruck
patent: 4621911 (1986-11-01), Lanni et al.
patent: 5218195 (1993-06-01), Hakamata
patent: 5323009 (1994-06-01), Harris
patent: 5386112 (1995-01-01), Dixon
patent: 5394268 (1995-02-01), Lanni et al.
patent: 5457536 (1995-10-01), Kornfield et al.
patent: 5477321 (1995-12-01), Johnson
patent: 5521705 (1996-05-01), Oldenbourg et al.
patent: 5521755 (1996-05-01), Stankewitz
patent: 5610765 (1997-03-01), Colucci
patent: 5672880 (1997-09-01), Kain
patent: 5719391 (1998-02-01), Kain
"Synthesis of Optical Birefrigerent Networks", Progress in Optics IX (North-Holland Amerstand) 1971, pp. 123-177 by E.O. Amman.
"Flat Passband Birefrigerent Wavelength Domain Multiplexer", Electronics Letters 23(3), 106-7 (1987) by W.J. Carlsen and C.F. Buhrer.
"Optical Network Synthesis Using Birefrigerent Crystals. I. Synthesis of Lossless Networks of Equal-Length Crystals", J. Opt. Soc. Am. 54, 1267 (1264) by S.E. Harris, E.O. Amman and C. Chang.
Hoyt Clifford C.
Miller Peter J.
Cambridge Research & Instrumentation Inc.
Evans F. L.
LandOfFree
Fluorescence imaging system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Fluorescence imaging system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescence imaging system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-472052