Fluorescence detection type gel electrophoresis apparatus

Details Fluorescence detection type gel electrophoresis apparatus Fluorescence detection type gel electrophoresis apparatus

1991-05-20

1992-03-31

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

250234, 356344, 36441301, B01D 6142, H01J 314, G01N 2100

Patent

active

051005298

ABSTRACT:
With respect to a gel which is mounted on a gel electrophoresis apparatus, a calibration process is provided before starting of measurement to obtain calibration data for bringing a portion of the gel irradiated with excitation light onto the optical axis of a light receiving optical system for each scanning point, to perform measurement while controlling relative positional relation between the optical path of the excitation light and the optical axis of the light receiving optical system through the calibration data. Displacement, which may be inevitably caused between the optical axis of the light receiving optical system and the position irradiated with the excitation light by errors in working, assembling and adjustment, is basically eliminated, and sensitiveness irregularity is also removed.

REFERENCES:
patent: 4130824 (1978-12-01), Amos et al.
patent: 4675095 (1987-06-01), Kambara
patent: 4726904 (1988-02-01), Ayers
patent: 4811218 (1989-03-01), Hunkapiller et al.
patent: 4812218 (1989-03-01), Hunkapiller et al.
patent: 4832815 (1989-05-01), Kambara et al.
patent: 4960999 (1990-10-01), McKean
patent: 4971677 (1990-11-01), Kambara et al.
European Search Report dated Oct. 4, 1991.

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