Fluorescence detection type electrophoresis apparatus

Details Fluorescence detection type electrophoresis apparatus Fluorescence detection type electrophoresis apparatus

1993-04-12

1994-03-01

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, 356344, G01N 2726, G01N 27447

Patent

active

052904199

ABSTRACT:
In the multi-color fluorescence detection type electrophoresis apparatus provided with the electrophoresis gel plate, excitation laser source, means to separate the fluorescence images according to each emission wavelength, and the detector of the fluorescence subjected to wavelength selection, two or more laser sources are provided, each of laser lights is irradiated on the sample on a time-sharing basis, and the filter which cuts off the scattered light of each the laser synchronous with the laser beam is installed in front of the wavelength separation means thereby providing simultaneous, quick and real-time analysis of a great number of samples such as DNA and RNA labeled by many types of fluorophores, without overlapping the wavelengths of the excitation light and the fluorescence.

REFERENCES:
patent: 4832815 (1989-05-01), Kambara et al.
patent: 5062942 (1991-11-01), Kambara et al.
Lloyd M. Smith et al "Fluorescence detection in automated DNA sequence analysis" Nature vol. 321 (1986) 674.varies.679.

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