Fluorescence detection

Details Fluorescence detection Fluorescence detection

2005-10-17

2010-10-12

Porta, David P (Department: 2884)

Radiant energy

Luminophor irradiation

With ultraviolet source

C250S461100, C250S484200, C435S287200

Reexamination Certificate

active

07812324

ABSTRACT:
A fluorescence detection system comprises a light source (22), dichroic mirror (32), excitation port (16), emission port (14), and a detector. The light source (22) is, for example, a pulsed ultraviolet LED, with a light emission that decays sufficiently rapidly to permit gated detection of fluorescence from a fluorescently-labelled species, at a time when it is distinguishable from autofluorescence. The detector is, for example, an electron multiplying CCD, with high gain on-chip amplification. A circuit (26) may be used to control a repeating cycle of (i) generation of a 20-200 microsecond UV. pulse; (ii) a gate delay of 1-5 microseconds; and (iii) a 10-800 microsecond detection period. This allows time-resolved-fluorescence-microscopy with real time or near real time operation.

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