Radiant energy – Luminophor irradiation – With ultraviolet source
Reexamination Certificate
2005-10-17
2010-10-12
Porta, David P (Department: 2884)
Radiant energy
Luminophor irradiation
With ultraviolet source
C250S461100, C250S484200, C435S287200
Reexamination Certificate
active
07812324
ABSTRACT:
A fluorescence detection system comprises a light source (22), dichroic mirror (32), excitation port (16), emission port (14), and a detector. The light source (22) is, for example, a pulsed ultraviolet LED, with a light emission that decays sufficiently rapidly to permit gated detection of fluorescence from a fluorescently-labelled species, at a time when it is distinguishable from autofluorescence. The detector is, for example, an electron multiplying CCD, with high gain on-chip amplification. A circuit (26) may be used to control a repeating cycle of (i) generation of a 20-200 microsecond UV. pulse; (ii) a gate delay of 1-5 microseconds; and (iii) a 10-800 microsecond detection period. This allows time-resolved-fluorescence-microscopy with real time or near real time operation.
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Connally Russell
Piper James Austin
Bryant Casey
Davis Paul
Goodwin & Procter LLP
Macquarie University
Porta David P
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