Fluorescence detecting device and fluorescence detecting method

Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Chemical analysis

Reexamination Certificate

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C356S032000

Reexamination Certificate

active

07822558

ABSTRACT:
A fluorescence detecting device irradiates an object to be measured with a laser beam, receives fluorescence generated from the object and processes a fluorescence signal generated when receiving the fluorescence. The device includes a laser light source section outputting the laser beam for irradiating the object, a light receiving section outputting the fluorescence signal of the fluorescence generated by the irradiated object, a light source control section generates a modulation signal having a frequency in order to time-modulate an intensity of the laser beam, and a processing section that calculates a fluorescence relaxation time of the fluorescence of the object based on the fluorescence signal output from the light receiving section. From the detection values acquired by the device including the phase information on the fluorescence, the intensity of the fluorescence is calculated.

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Non-patent document cited in the application: www://bdbiosciences.com/pharmingen/protocols/Fluorochrome—Absorption.shtml; Fluorochrome Absorption and Emission Spectra, pp. 1 to 7.
Keji, J., et al., “Flow Cytometric Characterization and Classification of Multiple Dual-Color Fluorescent Micropsheres Using Fluorescence Lifetime”, Cytometry, Jan. 1, 1998., vol. 33, No. 3. pp. 318-323.
European Search Report dated May 11, 2010.

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