Fluorescence-based scanning imaging device

Details Fluorescence-based scanning imaging device Fluorescence-based scanning imaging device

2011-03-22

2011-03-22

Consilvio, Mark (Department: 2872)

Optical: systems and elements

Deflection using a moving element

With particular mount or drive for element

C359S196100, C359S393000

Reexamination Certificate

active

07911670

ABSTRACT:
A device for analysing a specimen by fluorescence includes a confocal microscope, illumination means capable of emitting a light beam that converges, by means of an objective, on a focal spot, means for successively positioning the focal spot at various points on the specimen during analysis. The confocal microscope includes an objective mounted on a movable rapid-scan carriage driven in a reciprocating linear movement along a traverse direction by a rotating motor by means of a device of the connecting rod type. The specimen is placed on a movable support driven in a longitudinal movement and is able to move along the axis of the objective of the microscope in order to position the specimen relative to the focal spot. The excitation light spectrum is spread over the surface of the specimen in such a way that the excitation light reflected by the specimen and corresponding to the wavelengths close to fluorescence converge on points that are sufficiently distant from a diaphragm positioned in front of a device for measuring the fluorescence.

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