Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1997-07-24
1999-10-26
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C12Q 102
Patent
active
059726393
ABSTRACT:
A method is for determining the number of cells in cell culture is provided. In practicing the method, the cells are lysed and incubated at about 18.degree. C. to 25.degree. C. at acid pH with a benzothiazole substrate for a sufficient time to generate detectable fluorescence that is proportional to cell number, and then measuring fluorescence. The method is highly sensitive and can detect small differences or changes in cell number. Thus, the method can be used in drug screening assays. An automated high throughput screening assay for identifying compounds that modulate angiogenesis using the method is also provided.
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Moses et
Degen Nancy
IRORI
Larson Thomas G.
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