Fluorescence assays with improved sensitivity

Details Fluorescence assays with improved sensitivity Fluorescence assays with improved sensitivity

2009-04-21

2011-10-11

Seaman, D M (Department: 1625)

Organic compounds -- part of the class 532-570 series

Organic compounds

Chalcogen in the nitrogen containing substituent

C549S265000

Reexamination Certificate

active

08034928

ABSTRACT:
Latent fluorescent compounds, comprising a fluorescent molecule with one or more blocking groups attached and optionally one or more urea-containing groups are provided. The urea-containing group can be used to further attach one or more molecules of interest, such as proteins, peptides or nucleic acids. The blocking group(s) is released from the latent fluorescent compound by reaction with a trigger, forming the fluorescent molecule which can be detected. Also provided herein are methods of using latent fluorescent compounds to detect triggers.

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