Fluorescence assay of Ras farnesyl protein transferase

Details Fluorescence assay of Ras farnesyl protein transferase Fluorescence assay of Ras farnesyl protein transferase

1994-02-17

1996-06-11

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 4, 514 2, C12Q 148, C12Q 100, A01N 3718

Patent

active

055254799

ABSTRACT:
This invention is directed to a continuous fluorescence assay of Ras farnesyl:protein transferase. This assay can be used to screen for inhibitors of farnesyl:protein transferase. The assay may also be modified to screen for inhibitors of other protein transferases.

REFERENCES:
patent: 5043268 (1991-08-01), Stock
patent: 5135935 (1992-08-01), Alberts et al.
patent: 5141851 (1992-08-01), Brown et al.
Goldstein, J. L., et al., Nonfarnesylated Tetrapeptide Inhibitors of Protein Farnesyltransferase, (1991), Jour. of Biological Chemistry, 266, No. 24, pp. 15575-15578.
Schaber, M. D., et al., Polyisoprenylation of Ras in Vitro by a Farnesyl-Protein Transferase, (1990), Jour. Biol. Chemistry, 265, pp. 14701-14704.
Pompliano, D. L., et al., Intramolecular Fluorescence Enhancement: A Continuous Assay of Ras Farnesyl:Protein Transferase, (1992), J. Amer. Chem. Soc., 114, No. 20, pp. 7945-7946.
Reiss, Y., Purification of ras Farnesyl:Protein Transferase, (1990), Methods: A Companion to Methods in Enzymology, 1, No. 3, pp. 241-245.

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