Fluorescence assay for microbial beta-lactamase

Details Fluorescence assay for microbial beta-lactamase Fluorescence assay for microbial beta-lactamase

1984-08-06

1988-04-26

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 32, 435805, C12Q 134, C12Q 118

Patent

active

047404590

ABSTRACT:
A rapid method for the specific detection of the presence of Beta-lactamase from microbial sources is disclosed. The method utilizes a Beta-lactam ring containing substrate whose amide bond is hydrolyzed in the presence of Beta-Lactamase. A substrate, which includes a Beta-Lactam antibiotic with an acyl side chain containing an .alpha.-amino group and a .alpha.-phenyl group and/or its derivatives is first contacted with an organism thought to produce Beta-lactamase or a cell-free Beta-lactamase preparation, and, subsequently, it is determined whether the reaction product between the substrate and the organism or the preparation fluoresces.

REFERENCES:
patent: 4381343 (1983-04-01), Citri
Anderson, E. G. et al., J. Biol. Chem., vol. 258, No. 21, 1983, pp. 13120-13126.
Chen, K. C. S. et al., J. Clin. Micro., vol. 19, No. 6, 1984, pp. 818-825.
Wolfson, J. S. et al., Antimicrob. Agents and Chemother., vol. 23, 1983, pp. 308-312.
Bae, B. H. C. et al., J. Clin. Microb., vol. 17, 1983, pp. 545-547.

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