Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1991-03-12
1993-10-12
Nucker, Christine M.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
436536, 436537, 436538, 436514, 436517, 436800, 436805, 436807, 436810, 435 4, 435 71, 435 78, 435 79, 435803, 435808, 435968, 435971, G01N 3350, G01N 3353
Patent
active
052524922
ABSTRACT:
A method disclosed for studying the interaction in solution of two molecules of the type such as a ligand and a receptor that are capable of reacting or binding with each other. The method comprises preparing an aliquot of a solution containing the first of the molecules. The second of the molecules is then added to the aliquot. A fluorescently labeled molecule is added to the aliquot, wherein the fluorescently labeled molecule is also capable of reacting or binding with the second of the molecules. A porous matrix that is optically transparent is immersed into the aliquot containing the two molecules being studied and the fluorescently labeled molecule, wherein the second molecule and any fluorescently labeled molecule bound thereto is sterically hindered from permeating the porous, optically transparent matrix, while any unbound fluorescently labeled molecule permeates the matrix. The matrix that has been immersed into the aliquot is then viewed with means such as a fluorescent microscope for determining the amount of fluorescence emitted from the matrix.
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Crellin Terry M.
Dubrule Chris
Nucker Christine M.
University of Utah Research Foundation
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