Fluorescence assay for kinase activity

Details Fluorescence assay for kinase activity Fluorescence assay for kinase activity

2005-06-14

2005-06-14

Davis, Zinna Northington (Department: 1625)

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

C546S153000

Reexamination Certificate

active

06906194

ABSTRACT:
The present invention provides a sensor and methods for determining kinase activity.

REFERENCES:
Shultz et al, Journal of the American Chemical Society, vol. 125, No. 35, pp. 10591-10597, Apr. 2003.
Chien-An, C. et al., “Biosensors of Protein Kinase Action: From In Vitro Assays to Living Cells”,Biochimica et Biophysica Acta, 1697, pp. 39-51, 2004.
Chien-An, C. et al., “Design and Synthesis of a Fluorescent Reporter of Protein Kinase Activity”,J. Am. Chem. Soc., 124, pp. 3840-3841, 2002.
Higashi, H. et al., “Imaging of cAMP-Dependent Protein Kinase Activity in Living Neural Cells Using a Novel Fluorescent Substrate”,FEBS Letters, 414, pp. 55-60, 1997.
Hofmann, R.M. et al., “Fluorescent Monitoring of Kinase Activity in Real Time: Development of a Robust Fluorescence-Based Assay for Abl Tyrosine Kinase Activity”,Bioorganic&Medicinal Chemistry Letters, 11, pp. 3091-3094, 2001.
Kurokawa, K. et al., “A Pair of Fluorescent Resonance Energy Transfer-Based Probes for Tyrosine Phosphorylation of the CrkII Adaptor Protein in Vivo”,The Journal of Biological Chemistry, 276, No. 33, pp. 31305-31310, 2001.
Lawrence, D., “Chemical Probes of Signal-Transducing Proteins”,Acc. Chem. Res., 36, pp. 401-409, 2003.
McIlroy, B. K. et al., “A Continuous Fluorescence Assay for Protein Kinase C”,Analytical Biochemistry, 195, pp. 148-152, 1991.
Nagai, Y. et al., “A Fluorescent Indicator for Visualizing cAMP-Induced Phosphorylation in Vivo”,Nature America Inc., 18, pp. 313-316, 2000.
Post, P. et al., “A Genetically Engineered, Protein-Based Optical Biosensor of Myosin II Regulatory Light Chain Phosphorylation”,The Journal of Biological Chemistry, 269 No. 17, pp. 12880-12887, 1994.
Sato, M. et al., “Fluorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells”,Nature Biotechnology, 20, pp. 287-294, 2002.
Shults, M.D. et al., “Versatile Fluorescence Probes of Protein Kinase Activity”,J. Am. Chem. Soc., 125, pp. 14284-14249, 2003.
Ting, A. Y. et al., “Genetically Encoded Fluorescent Reporters of Protein Tyrosine Kinase Activities in Living Cells”,PNAS, 98, No. 26, pp. 15003-15008, 2001.
Violin, J.D. et al., “A Genetically Encoded Fluorescent Reporter Reveals Oscillatory Phosphorylation by Protein Kinase C”,The Journal of Cell Biology, 161, No. 5, pp. 899-909, 2003.
Wright, D. E. et al., “Fluorometric Assay for Adenosine $3^{\prime}$,$5^{\prime}$-Cyclic Monophosphate-Dependent Protein Kinase and Phosphoprotein Phosphatase Activities”,Proceedings of the National Academy of Sciences of the United States of America, 78, No. 10, pp. 6048-6050, 1981.
Yeh, R. et al., “Real Time Visualization of Protein Kinase Activity in Living Cells”,The Journal of Biological Chemistry, 277 No. 13, pp. 11527-11532, 2002.
Zhang, J. et al., “Genetically Encoded Reporters of Protein Kinase A Activity Reveal Impact of Substrate Tethering”,PNAS, 98, No. 26, pp. 14997-15002, 2001.

Affiliated with

Also associated with

Rating

Fluorescence assay for kinase activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescence assay for kinase activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescence assay for kinase activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3502105