Fluorescence and motility characterization system for cells, bac

Image analysis – Histogram processing – For setting a threshold

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382 1, 356 39, 3644131, G06K 900

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050938665

ABSTRACT:
An apparatus and method for determining motility and other characteristics of cells in a fluid medium employing both the scattering and transmission of light through that medium and the absorption of shorter wavelength light by the cell with subsequent emission of fluorescent light. Both forms of light are imaged on an image detection apparatus, the output of which is analyzed as a function of time to produce the information concerning the characteristics of the cell.

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Leif et al., "Development of Instrumentation and Fluorchromes for Automated Multiparameter Analysis of Cells", Clinical Chemistry, vol. 23, No. 8, 1977, pp. 1492-1498.
Kucera et al., "Computer-Controlled Double-Beam Scanning Microspectrophotometry for Rapid Microscopic Image Reconstructions", J. of Microscopy, vol. 116, 7/79, pp. 173-184.

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