Optics: measuring and testing – Blood analysis
Patent
1978-07-13
1981-01-06
McGraw, Vincent P.
Optics: measuring and testing
Blood analysis
356 73, 356318, 250461B, G01J 2164
Patent
active
042433185
ABSTRACT:
In fluorescence analysis of multiply stained particles, particularly biological cells, in a continuous flowthrough procedure, in which the particles are suspended in a carrier fluid, the carrier fluid containing the suspended particles is encased in a sheath stream, the resulting composite stream is conducted in laminar flow through an intensive laser light zone where fluorescent light pulses are generated by the action of the laser light on the particles and emanate from the particles, and those fluorescent pulses are detected and processed in real time in an electronic evaluation system, the laser light zone is produced by focussing at least two laser beams of respectively different wavelengths on two points spaced at a given distance apart along the path of the particle-containing fluid in the composite stream, and the detection and processing is effected by correlating the fluorescence pulses emanating from the two points on the basis of the spacing between the two points and of the flow speed of the stream, in order to evaluate only those pulses which correspond to the travel time of individual particles between the two points.
REFERENCES:
patent: 3916197 (1975-10-01), Fulwyler
patent: 3976862 (1976-08-01), Curbelo
Deutsches Krebsforschungszentrum
McGraw Vincent P.
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