Fluorescein labelled phosphoramidites

Details Fluorescein labelled phosphoramidites Fluorescein labelled phosphoramidites

1994-12-02

1996-12-10

Kunz, Gary L.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

549223, 549225, 536 243, C07D31178, A61K 31335

Patent

active

055832369

ABSTRACT:
Compounds useful to attach a fluorescein label to an oligonucleotide are disclosed. To create these compounds, oxygen groups on the fluorescein moiety are protected (e.g. with acyl groups). One then links a phosphoramidite to the fluorescein moiety via an amide or thiourea linkage to an active site on the fluorescein ring. The resulting compound can then be directly linked to an oligonucleotide as it is being formed in an automated synthesizer and then deprotected using the same deprotection conditions as are used to deprotect the oligonucleotides. Such compounds are, inter alia, useful to create labelled primers for DNA sequencing.

REFERENCES:
patent: 4762779 (1988-08-01), Snitman
Schubert, et al., Nucl. Acids Res. 18[11]:3427 (1990).
Trainor, et al., "Chemical Reagents for Molecular Biology", Apr. 6, 1989 (Abstract).
Roget, et al., Nucl. Acids Res. 17[19]:7643-7651 (1989).
Alves, et al., Tetrahedron Letters 30[23]:3089-3092 (1989).
Bannwarth, et al., Tetrahedron Letters 30[12]:1513-1516 (1989).
Misiura, et al., Nucl. Acids Res. 18[15]:4345-4345 (1990).
"TFA Aminolinker Amidite", Pharmacia LKB Biotechnology advertisement.
"AutoPrimer Synthesis Kit Instructions", booklet from Pharmacia LKB Biotechnology (1990).
Haralambidis, et al., Tetrahedron Letters 28[43]:5199-5202 (1987).
Pieles, et al., Nucl. Acids Res. 18[15]:4355-4360 (1990).
Haugland, Handbook of Fluorescent Probes and Research Chemicals, pp. 30-32, 34-36 (1989).
Smith, et al., Nature 321:674-679 (1986).
Ansorge, et al., Nucl. Acids Res. 15[11]:4593-4602 (1987).
Kaiser, et al., Nucl. Acids Res. 17[15]:6087-6102 (1989).
Voss, et al., Nucl. Acids Res. 17[7]:2517-2527 (1989).
Ansorge, et al., J. Biochem. & Biophys. Meth. 13:315-323 (1986).
Schulhof, et al., Nucl. Acids Res. 15[2]:397-416 (1987).
Abstracts of Papers (Part 1), Abstract No. 35, 203rd American Chemical Society National Meeting, Apr. 1992.
Millipore Product Bulletin for FluorDite, May 1993.
P. 118 from 1993-1994 Applied Biosystems catalog showing FAM Amidite.
Cohen, et al., "Use of phosphoramidite-linked acridine for fluroescent tagging of oligo-deoxy-nucleotides", Derwent World Patents Index Accession No. 89-129850/17 (1989).
"TFA Aminolinker Amidite", Pharmacia LKB Biotechnology advertisement (Nov. 1990).

Affiliated with

Also associated with

Rating

Fluorescein labelled phosphoramidites does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Fluorescein labelled phosphoramidites, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Fluorescein labelled phosphoramidites will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-425056