Fluidic temperature gradient focusing

Details Fluidic temperature gradient focusing Fluidic temperature gradient focusing

2006-04-18

2006-04-18

Noguerola, Alex (Department: 1753)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S601000, C204S450000, C204S600000

Reexamination Certificate

active

07029561

ABSTRACT:
A method and device are provided for concentrating and separating ionic species in solution within a fluidic device having a fluid conduit such as a channel or capillary. The concentration is achieved by balancing the electrophoretic velocity of an analyte against the bulk flow of solution in the presence of a temperature gradient. Using an appropriate buffer, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity so that the electrophoretic and bulk velocities sum to zero at a unique point and the analyte will be focused at that point. The method and device may be adapted for use with a variety of analytes including fluorescent dyes, amino acids, proteins, DNA and to concentrate a dilute analyte.

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