Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1977-03-11
1978-07-18
Prescott, Arthur C.
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726
Patent
active
041014014
ABSTRACT:
Electrophoretic separation of samples in a running gel is obtained by depositing three layers of liquid in an outer container. The liquid layers have increasing specific gravities proceding from the uppermost to the lowermost layer. The upper and lower layers are electrically conductive buffer solutions which are immiscible with the center layer which is electrically insulating. A rack is provided for holding running gels in the outer container so that the upper end of the gels are in contact with the upper buffer layer and the lower end of the gels are in contact with the lower buffer layer. An upper and a lower electrode is provided in contact with each of the upper and lower buffer solutions respectively. A voltage impressed between the upper and lower electrodes provides a substantially unidirectional electrical field through the running gel and separation of samples in the running gel substantially free of distortion due to the absence of spurious electrical fields at the sides of the running gel adjacent to the central layer of electrically insulating fluid. Removal of the heat of electrophoresis and further reduction of distortion in separated samples is achieved by cooling the layered liquids using an immersed cooling coil or by recirculating the central layer through a heat exchanger.
REFERENCES:
patent: 3129158 (1964-04-01), Raymond et al.
patent: 3506554 (1970-04-01), Broome
patent: 3563880 (1971-02-01), Anderson
patent: 3719580 (1973-03-01), Roberts et al.
patent: 3902986 (1975-09-01), Nees
patent: 3932265 (1976-01-01), Hoefer
patent: 3980546 (1976-09-01), Caccavo
Hoefer Scientific Instruments
Prescott Arthur C.
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