Fluid analysis

Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids

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Details

436 8, 436 79, 436166, G01N 3320, G01N 3352

Patent

active

044488890

ABSTRACT:
In a method for colorimetric determination of calcium, the sample to be analyzed is mixed with a dye reagent of pH value at which that reagent does not produce a predetermined change when complexed with calcium ions; a first colorimetric measurement is made on the mixture of the sample and dye reagent; a second reagent is added to the mixture of the sample and dye reagent to shift the pH and activate the dye reagent producing the predetermined change; and then a second colorimetric measurement is made on the resulting mixture, the difference between the two colorimetric measurements being a measure of calcium in the sample.

REFERENCES:
patent: 3121613 (1964-02-01), Bittner
patent: 3457045 (1969-07-01), Fraguada et al.
patent: 3754865 (1973-08-01), Gindler
patent: 3771961 (1973-11-01), Denney
patent: 3798000 (1974-03-01), Helger
patent: 3822116 (1974-07-01), Morin
patent: 3854889 (1974-12-01), Rathje
patent: 3874853 (1975-04-01), Byrnes
patent: 3934977 (1976-01-01), Cleaver
patent: 3938954 (1976-02-01), Stavropoulos et al.
patent: 4308027 (1981-12-01), Ceriotti
patent: 4329149 (1982-05-01), Schoonover et al.
Olthuis et al., Interference of Free Fatty Acids with the Determination of Calcium in Serum, "Clinica Chimica Acta", 49 (1973), pp. 123-124.
Ladenson et al., Multiple Myeloma and Hypercalcemia?, "Clinical Chemistry", vol. 25, No. 10, (1979) pp. 1821-1825.

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