Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2008-05-13
2008-05-13
Bertoglio, Valarie (Department: 1632)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S325000
Reexamination Certificate
active
11184156
ABSTRACT:
A gene activation/inactivation and site-specific integration system has been developed for mammalian cells. The invention system is based on the recombination of transfected sequences by FLP, a recombinase derived fromSaccharomyces. In several cell lines, FLP has been shown to rapidly and precisely recombine copies of its specific target sequence. For example, a chromosomally integrated, silent β-galactosidase reporter gene was activated for expression by FLP-mediated removal of intervening sequences to generate clones of marked cells. Alternatively, the reverse reaction can be used to target transfected DNA to specific chromosomal sites. These results demonstrate that FLP can be used, for example, to mosaically activate or inactivate transgenes for a variety of therapeutic purposes, as well as for analysis of vertebrate development. The FLP recombination system of the present invention can be incorporated in transgenic, non-human mammals to achieve site-specific integration of transgenes, to construct functional genes or to disrupt existing genes.
REFERENCES:
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Mullins, 1990; Nature, vol. 344, pp. 541-544.
Hammer, 1990, Cell, vol. 63, 1099-1112.
Mullins, 1989, EMBO J., vol. 8, pp. 4065-4072.
Taurog, 1988, Jour. Immunol., vol. 141, pp. 4020-4023.
Wall, 1996, Theriogenology, vol. 45, pp. 57-68.
Overbeek, 1994, “Factors affecting transgenic animal production,” Transgenic animal technology, pp. 96-98).
Mullins, 1996, J. Clin. Invest., vol. 98, pp. S37-S40.
Golic, 1989, Cell, vol. 59, pp. 499-509.
Dymecki, 1996, PNAS, vol. 93, pp. 6191-6196.
Le Mouellic, 1990, PNAS, vol. 87, pp. 4712-4716.
Awatramani, 2001, Nature Gnetics, vol. 29, pp. 257-259.
Soriano, 1999, Nature Gnetics, vol. 21, pp. 70-71.
O'Gorman Stephen V.
Wahl Geoffrey M.
Bertoglio Valarie
Foley & Lardner LLP
Reiter Stephen E.
The Salk Institute for Biological Studies
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