Flow-through electroporation system for ex vivo gene therapy

Details Flow-through electroporation system for ex vivo gene therapy Flow-through electroporation system for ex vivo gene therapy

1998-06-03

2000-02-22

McDermott, Corrine

Surgery

Means for introducing or removing material from body for...

Treating material introduced into or removed from body...

4351736, 604 4, A61M 3700, A61M 3100, C12N 1300

Patent

active

060274883

ABSTRACT:
A method and apparatus for introducing a preselected molecule into a living cell by contacting the cell with the preselected molecule and applying a multiple series of electrical pulses to the cell. The method can be utilized ex vivo. The multiple electrical pulses generate rotating electric fields which introduce transient pores in the living cell without killing the cell. The rotating electric fields are provided in a flow through chamber apparatus having more than two electrodes. A three-step pulse process, e.g. collection, electroporation, electrophoresis, is used to introduce the preselected molecule into the cell. A mechanical means of repositioning cells between successive pulses is also provided. The apparatus can also provide a means to pulse cells at different temperatures and then after pulsing, let the cells recover for a specified residence time at another temperature.

REFERENCES:
patent: 5545130 (1996-08-01), Hofmann et al.
patent: 5612207 (1997-03-01), Nicolau et al.
patent: 5676646 (1997-10-01), Hofmann et al.
patent: 5702359 (1997-12-01), Hofmann et al.
patent: 5729201 (1998-03-01), Meserol

Affiliated with

Also associated with

Rating

Flow-through electroporation system for ex vivo gene therapy does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Flow-through electroporation system for ex vivo gene therapy, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Flow-through electroporation system for ex vivo gene therapy will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-516804