Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1990-02-23
1993-02-02
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 733, 435 734, 435 736, 435 792, 435 793, 435961, 435968, 436518, 436529, 436538, 436541, 422 70, 422 82, 422 8205, G01N 3353, G01N 33569
Patent
active
051837404
ABSTRACT:
A method of detecting a target moiety comprising the steps of (a) providing an antibody specific to the target, (b) saturating the binding sites of the antibody with a labelled form of the target, (c) flowing a liquid containing the target past the saturated antibody, thereby (d) allowing the target to displace the labelled antigen, and (e) detecting the displaced labelled antigen with a detector for the label.
Also, the invention is accomplished by a flow immunosensor comprising: a liquid flowing through the immunosensor for moving a sample through the immunosensor; a receiving means for introducing the sample to the liquid stream; a flow control means for moving the liquid stream through the immunosensor; an exchanger connected to the sample receiving means in which the sample is brought in contact with labelled antigens of the target and whereby any target present is allowed to displace the labelled antigen; a detection apparatus connected to the exchanger for detecting any labelled antigen which is released; and a disposal means connected to the detection apparatus for disposing of or collecting of waste coming from the detection apparatus.
REFERENCES:
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patent: 4582809 (1986-04-01), Block et al.
patent: 4803170 (1989-02-01), Stanton et al.
patent: 4895809 (1990-01-01), Schlabach et al.
E. Maggio (ed), Enzyme-Immunoassay, (CRC Press, Inc. Boca Ratio, Fla.) pp. 24-233, 243, 289 (1980).
Kelly, "Separation-Free Enzyme Fluorescence Immunoassay by Continuous Flow Injection Analysis" in Enzyme-Mediated Immunoassay, (Plenum Press 1985) 191-201.
Kusterbeck Anne W.
Ligler Frances S.
Wemhoff Gregory A.
Edelberg Barry A.
Kepplinger Esther L.
McDonnell Thomas E.
Spiegel Carol A.
The United States of America as represented by the Secretary of
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