Flow cytometry lytic agent and method enabling 5-part leukocyte

Details Flow cytometry lytic agent and method enabling 5-part leukocyte Flow cytometry lytic agent and method enabling 5-part leukocyte

1992-08-24

1996-04-23

Warden, Robert J.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 10, 356 39, G01N 3348

Patent

active

055102670

ABSTRACT:
A lytic agent is described which when mixed with whole blood, lyses the red cells instantly, but does not affect the optical, light scattering properties of the white blood cells for at least 30 seconds. This lytic agent enables the performance of a five part leukocyte differential by flow cytometry and light scattering. In its preferred embodiment, the lytic agent incorporates 2-phenoxyethanol which combines the functions of leukoprotective and antimicrobial, TRITON X-100 (octylphenoxypolyethoxyethanol) a lytic and wetting agent and TRIS/HCl to provide pH buffering and to increase the solution's conductivity.

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