Flow cytometry-fluorescence measurements for characterizing sper

Details Flow cytometry-fluorescence measurements for characterizing sper Flow cytometry-fluorescence measurements for characterizing sper

1982-09-01

1985-12-17

Turk, Arnold

Chemistry: analytical and immunological testing

Biological cellular material tested

2504612, 436 94, 436172, G01N 2164, G01N 3352

Patent

active

045593094

ABSTRACT:
A process for characterizing sperm motility and viability by staining a sperm sample with Rhodamine 123 and ethidium bromide, and simultaneously measuring the sperm fluorescence emissions at green frequencies 515-575 nm and at red frequencies 600-650 nm, the green counts being correlated with sperm motility and the red counts being correlated with putative dying or dead cells. Additionally, a sample of sperm can be characterized as to type and normality by staining a sample of sperm with acridine orange and simultaneously measuring the sperm fluorescence emissions at green frequencies 515-575 nm and at red frequencies 600-650 nm.

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