Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-09-03
1998-06-09
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 724, 435975, 436507, G01N 3353
Patent
active
057632014
ABSTRACT:
The present invention relates to a flow cytometry assay for the determination of heparin-induced thrombocytopenia. This method (a) detects in a plasma sample the presence of anti-heparin antibodies which react with platelets in the presence of heparin to produce activated platelets, (b) quantitates by flow cytometry the presence of such activated platelets in the plasma sample, and (c) correlates the presence of activated platelets with a diagnosis of HIT for the patient. In addition, the flow cytometry assay of the invention is also useful in assessing the compatibility of a heparin-like molecule for use as an alternate therapy for patients with heparin-induced thrombocytopenia. In addition, the invention contemplates a mepacrine release assay with flow cytometry for the detection of heparin-induced thrombocytopenia and for the assessment of compatibility of heparin-like molecules in patients diagnosed with heparin-induced thrombocytopenia.
REFERENCES:
patent: 5246832 (1993-09-01), Michelson et al.
patent: 5466582 (1995-11-01), Amiral
patent: 5529902 (1996-06-01), Kottke et al.
patent: 5627036 (1997-05-01), Rsutelingsperger
Information sheet on LOVENOX (enoxaparin sodium) injection, Rhone-Poulenc Rorer Pharmaceuticals Inc., Collegeville, Pa.
Kelton, J.G. et al., "Clinical usefulness of testing for a heparin-dependent platelet-aggregating factor in patients with suspected heparin-associated thrombocytopenia," J. Lab. Clin. Med., (1984) 103(4):606-612.
Sheridan, D. et al., "A Diagnostic Test for Heparin-Induced Thrombocytopenia," Blood (1986) 67(1):27-30.
George, J.N. et al., "Platelets", Section III: Hemostasis, Hematology 1994, Education Program, Am. Soc. Hematology, (Dec. 2-6, 1994), pp. 66-74.
McEver, R.P. and Martin, M.N., "A Monoclonal Antibody to a Membrane Glycoprotein Binds Only to Activated Platelets," J. Biol. Chem., (1984) 259(15):9799-9804.
Shattil, S.J. et al., "Changes in the Platelet Membrane Glycoprotein llb.cndot.IIIa Complex during Platelet Activation," J. Biol. Chem. (1985) 260(20):11107-11114.
Thiagarajan, P. and Tait, J.F., "Binding of Annexin V/Placental Anticoagulant Protein I to Platelets," J. Biol. Chem. (1990) 265(29):17420-17423.
Tomer, A., "Reduced Predisposition to Thrombosis in Sickle Cell Disease (SCD) Patients Treated with Dietary n-3 Fatty Acids (n-3FA)," Blood (1995) 86(10), Suppl. 1:1182.
Wall, J.E. et al., "A flow cytometric assay using mepacrine for study of uptake and release of platelet dense granule contents," British J. Haematology (1995) 89:380-385.
Kelton et al, Blood, 83, 3232-3239, 1994.
Lee et al, Brit. Jour. Haemat., 95, 724-731, 1996.
Emory University
Saunders David
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