Flow cytometric, whole blood dendritic cell immune function...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200, C435S040500, C435S287200, C435S962000, C435S973000, C436S010000, C436S017000, C436S063000, C436S064000, C436S164000, C436S172000, C436S177000, C436S522000, C436S546000

Reexamination Certificate

active

07351546

ABSTRACT:
The invention provides a flow cytometric method for measuring dendritic cell function in whole blood, comprising: (a) contacting a whole blood sample with a dendritic cell activator; (b) adding to the sample a plurality of dendritic cell-distinguishing antibodies and at least one cytokine-specific antibody; and then (c) flow cytometrically assaying the sample for the binding of the cytokine-specific antibody by at least one distinguishable DC subset. Other assays are presented in which DC activation markers are assessed.

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