Drug – bio-affecting and body treating compositions – Lymphokine – Interferon
Patent
1995-11-17
1998-04-07
Walsh, Stephen
Drug, bio-affecting and body treating compositions
Lymphokine
Interferon
424 851, 424 852, 424 855, 424 856, 435 4, 435 6, 435 29, 435 34, 435 39, 435 405, 435325, 435347, 436172, A61K 3819, C12Q 106, G01N 3352
Patent
active
057361290
ABSTRACT:
A method of treating cancer by the use of a multidrug chemotherapeutic regimen determined by in vitro pharmacosensitivity tests. A cell suspension is prepared from a tumor specimen obtained from the patient. The viable tumor cell count within the cell suspension is calculated. The volume of the cell suspension is then adjusted to obtain a base cell concentration by diluting the cell suspension with patient medium in proportion with the viable tumor cell count. A sample of the cell suspension is retained as a negative control sample. Drug samples are then prepared, each drug sample containing a mixture of cell suspension, patient medium, and a drug selected from several drugs, wherein each drug sample contains a different drug which is added to the drug sample in an aliquot amount proportional to the base cell concentration. The drug samples and negative control sample are then incubated. After incubation, the drug samples and negative control sample are stained with a DNA intercalating dye. The cell viability in the drug samples and negative control sample is determined by use of a flow cytometer. The cell viability in the drug samples and negative control sample is compared to determine the pharmacosensitivity of the tumor. A multidrug treatment regimen is then administered to the patient, wherein the regimen includes the drugs shown to be most effective against the tumor in the pharmacosensitivity assay. The treatment has been shown to be especially useful in the simultaneous treatment of primary tumors and their metastases, especially when the chemotherapeutic regimen is administered locoregionally by intra-arterial infusion methods.
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Medenica Rajko D.
Powell David K.
Pak Michael D.
Walsh Stephen
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