Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1984-06-05
1988-10-25
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 7, 435 29, 435 91, 436 56, 436 63, 436 94, 436800, 935 76, 935 77, C12Q 168
Patent
active
047804068
ABSTRACT:
A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide or Hoechst 33258 is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as halodeoxy-uridine (HdU), more specifically bromodeoxyuridine (BrdU) is used as a probe for the measurement of HdU or BrdU uptake by the cells as a measure of DNA synthesis.
REFERENCES:
Schwartz, Biochemistry, vol. 15, no. 14, (1976) pp. 3097-3105.
Dolbeare, et al., Clin. Chem., vol. 23, No. 8, (1977), pp. 1485-1491.
Gratzner, Science, vol. 218, (1982), pp. 474-475.
Gratzner, et al., Cytometry, vol. 1, No. 6, (1981), pp. 385-389.
Noguchi, et al., Cytometry, vol. 1, No. 6, (1981), pp. 390-393.
Gordon, et al., J. Immunol., vol. 127, No. 4 (1981), pp. 1634-1639.
Dolbeare Frank A.
Gray Joe W.
Macevicz Stephen C.
Nucker Christine M.
Rajender Shyamala T.
The Regents of the University of California
Weis Berthold J.
LandOfFree
Flow cytometric measurement of total DNA and incorporated halode does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Flow cytometric measurement of total DNA and incorporated halode, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Flow cytometric measurement of total DNA and incorporated halode will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2269269