Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1983-10-18
1986-04-29
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 7, 435 29, 436 63, 436 94, 935 77, 935108, C12Q 168, G01N 3353, G01N 3348
Patent
active
045857369
ABSTRACT:
A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.
REFERENCES:
patent: 4196265 (1980-04-01), Koprowski et al.
Barraco et al., Cancer Res., 42 (1982), 2894-2898.
Pallavicini et al., Cancer. Res., 42 (1982), 3125-3131.
Hart et al., Cancer, 39 (1977), 1603-1617.
Van Dilla et al., Science, 163 (1969), 1213-1214.
Gratzner, Science, 218 (1982), 474-475.
Quastler et al., Exp. Cell Res., 17 (1959), 420-438.
Gray et al., Cell Tissue Kinet., 10 (1977), 97-109.
Gratzner et al., Exp. Cell. Res. 95 (1975), 88-94.
Gratzner et al., T. Histochem. Cytochem., 24 (1976), 34-39.
Gratzner et al., Res. Comm. Chem. Pathol. Pharmacol. 20 (1978), 539-548.
Gray et al., J. Cell. Physiol., 108 (1981), 135-144.
Gray, Pharmac Ther., 22 (1983), 163-197.
Dean et al., UCRL preprint #89715, Aug. 12, 1983.
Dolbeare et al., UCRL preprint #88643, Jan. 19, 1983.
Dolbeare et al., Proc. Natl. Acad. Sci. USA, 80(18), 5573-5577 (1983).
Noguchi et al., Chemical Abstracts, 98 (1983), #85652.
Gordon et al., J. Immunol., 127 (1981) 1634-1639.
Gratzner et al., Cytometry, 1 (1981), 385-9.
Shulman et al., Nature, Nov. 16, 1975, pp. 269-270.
Dolbeare Frank A.
Gray Joe W.
Clouse, Jr. Clifton E.
Hightower Judson R.
Nucker Christine M.
Rajender Shyamala
The United States of America as represented by the United States
LandOfFree
Flow cytometric measurement of total DNA and incorporated halode does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Flow cytometric measurement of total DNA and incorporated halode, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Flow cytometric measurement of total DNA and incorporated halode will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-141646