Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-08-01
2006-08-01
Sisson, Bradley L. (Department: 1634)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C435S287200, C536S024300, C536S024330
Reexamination Certificate
active
07083951
ABSTRACT:
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5′ end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3′ end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5′ end.
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Langlois Richard G.
Nasarabadi Shanavaz
Venkateswaran Kodumudi S.
Lee Ann M.
Lee John H.
Sisson Bradley L.
The Regents of the University of California
Thompson Alan H.
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