Flow cytometric apparatus and method

Measuring and testing – Sampler – sample handling – etc. – Capture device

Reexamination Certificate

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Reexamination Certificate

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07658121

ABSTRACT:
The present invention discloses a flow cytometric apparatus comprising a flow chamber to be passed through by a sample in a row in sequence, a sample storage means for metering and storing the sample, a sample syringe for driving the sample into the flow chamber, a syringe-type injection-suction means comprising a sheath liquid syringe and a liquid-path switching means connected with it, and a control unit for controlling the operations of the sample syringe and the syringe-type the injection-suction means. In a sample charging stage, the injection-suction means feeds the sample into the sample storage means so that the sample is temporarily stored in it in a ready-to-use state, and in a sheath-liquid injection stage, the injection-suction means feeds the sheath liquid into the flow chamber to form a sheath flow. A flow cytometric method performed by the flow cytometric apparatus is also disclosed.

REFERENCES:
patent: 5134445 (1992-07-01), Toge
patent: 5182617 (1993-01-01), Yoneyama et al.
patent: 5895764 (1999-04-01), Sklar et al.
patent: 1204267 (2005-06-01), None
patent: 01000306 (2007-07-01), None
patent: 1348943 (2003-10-01), None
patent: 6034518 (1994-02-01), None
patent: 6194299 (1994-07-01), None
patent: 2001296233 (2001-10-01), None
Chinese Search Report dated Aug. 8, 2007 for China patent application No. 200710074105.1.
Yi, Zhang, “The Introduction to the Flow Cytometer”, China Academic Journal electronic Publishing House, Aug. 2005, p. 25-26.
“Principle of Flow Cytometry”, China Academic Journal Electronic Publishing House, Article No. 1004-2113 (2002) 03-0203-0205.
English translation of abstracts for EP1348943A, JP6034518A, JP6194299A, CN1204267C, CN101000306A and two articles reference above.

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