Flow cytometer for identifying algae by chlorophyll fluorescence

Details Flow cytometer for identifying algae by chlorophyll fluorescence Flow cytometer for identifying algae by chlorophyll fluorescence

1982-03-22

1985-02-19

Naff, David M.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

356318, 435 4, 435 29, 435 34, 435257, C12M 134, C12Q 129, C12Q 104, G01J 330

Patent

active

045006415

ABSTRACT:
Identification of algae in water samples is carried out with a flow cytometer having at least one high intensity light source to provide a light path such as a laser, means for passing the algae through the light path and a system of detectors arranged to measure chlorophyll fluorescence and at least two other parameters such as forward light scattering, perpendicular light scattering, backward light scattering, pulse length and/or shape of the scattered signals, fluorescence and pulse length and/or shape of the fluorscence signals. In order to separate the light output from the flow cytometer into components which can yield useful measurements, a system of partially reflecting mirrors and dichroic mirrors is used. Preferably, the high intensity light source is two independent lasers having parallel light paths one of which is separated in a UV range and the other in an all lines mode.

REFERENCES:
patent: 3525803 (1970-08-01), Smart
patent: 4043669 (1977-08-01), Gehatia et al.
patent: 4061543 (1977-12-01), Bean et al.
patent: 4101383 (1978-07-01), Wyatt et al.
patent: 4203670 (1980-05-01), Bromberg

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