Flow cytometer calibration method

Details Flow cytometer calibration method Flow cytometer calibration method

1995-12-27

1998-11-17

Pyon, Harold Y.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

436 8, 436800, 435967, 356 42, 356243, G01N 33547

Patent

active

058375473

ABSTRACT:
A method for calibrating a flow cytometer or fluorescence microscope and for quantitating binding proteins, such as antibodies. Populations of microbeads are provided which are in equilibrium with a saturating amount of fluorescently-labeled protein. The microbeads may have an incorporated fluorescent dye which has a strong signal in a selected channel of a flow cytometer, but no signal in other channels.

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