Flow chamber and electro-manipulator incorporating same

Details Flow chamber and electro-manipulator incorporating same Flow chamber and electro-manipulator incorporating same

1987-12-15

1989-01-24

Scott, Samuel

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435173, 417118, 417567, 417568, 204299R, 2041801, 935 93, 935 94, 935 89, C12M 100, C12N 1300

Patent

active

048001635

ABSTRACT:
A sample stored in a reservoir is introduced into a flow chamber having a pair of panel electrodes interposed by an insulating flat plate spacer having an opening. After electro-fusion is completed or while electro-transfection is being conducted by the application of a suitable voltage between the panel electrodes, fused cells or cells that have been transfected with nucleic acids are discharged out of the flow chamber, and the sample in the reservoir is simultaneously introduced into the flow chamber. This cycle of operations is repeated.

REFERENCES:
patent: 3078868 (1963-02-01), Lamb
patent: 3175688 (1965-03-01), Zink
patent: 3460810 (1969-08-01), Moeller
patent: 4441972 (1984-04-01), Pohl
patent: 4578167 (1986-03-01), Schoner
patent: 4695547 (1987-09-01), Hilliard et al.
Physiol Plant 67: 507-516, Copenhagen 1986, Zachrisson et al., Electromanipulation of Plant Protoplast.
J.gen Virol (1986), 67: 2037-2042, Hibi et al., High Efficiency Electro-Transfection of Tobacco Mesophyll Proplasts with Tobacco Mosaic Virus RNA.
Angew.Chem. Int. Ed Engl. 20. 325-344, 1981, Zimmermann et al., Cells with Manipulated Funtions: New Perspectives for Cell Biology, Medicine, and Technology.

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