Flow cell SELEX

Details Flow cell SELEX Flow cell SELEX

1997-01-31

1999-01-19

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 254, 935 77, 935 78, 422 8811, C12P 1934, C12Q 168, C07H 2102, G01N 2101

Patent

active

058612548

ABSTRACT:
Described herein are methods for improved partitioning between high and low affinity nucleic acid ligands identified through the SELEX method, termed Flow Cell SELEX. The Flow Cell SELEX method achieves partitioning between high and low affinity nucleic acid ligands using surface plasmon resonance technology. The method of the present invention presents a new and powerful approach to select nucleic acid ligands by providing a partitioning method which 1) enables a significant increase in the efficiency of partitioning from traditional partitioning methods used in SELEX, 2) allows for real time monitoring of the partitioning of the high affinity ligands from the low affinity ligands 3) allows for the ability to select for a nucleic acid ligand having specific kinetic properties, 4) does not rely on radiolabeling or other means of tagging for detection, and 5) allows for use of smaller amounts of target than in traditional methods of SELEX.

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