Flexion enhanced transfection of genetic material

Details Flexion enhanced transfection of genetic material Flexion enhanced transfection of genetic material

1994-11-18

1996-05-21

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351736, 435174, 4352402, 435240241, C12N 1564

Patent

active

055189091

ABSTRACT:
A method of enhancing transfection of genetic material by subjecting the cells to be transfected to elongation or flexion. Preferably, the elongation or flexion is cyclical. Improved efficiency of transfection of genetic material is demonstrated in cells subjected to elongation or flexion as compared to the same transfection of the same cells under quiescent conditions. Flexion preferably takes place on a cell or cells in culture wherein the culture is adhered to a rubber-based culture well, and the rubber-based well is subjected to cyclical flexion at a rate between 100 cycles per second and 20 cycles per minute, at 0.001-25% cell elongation based on the diameter of the cell. More preferably, flexion is conducted at 60 cycles per minute for 15 minutes to 12 hours, at a maximum cellular elongation of 5-20% of the cell diameter in either a strain gradient that is maximal at the culture well periphery, minimal at the center and nominally biaxial at the periphery but up to 50% biaxial toward the well center, or is a more homogeneous biaxial load across 75% to 85% of the culture well surface.

REFERENCES:
patent: 4789601 (1988-12-01), Banes
A Concise Dictionary of Biology, 1990, Oxford University Press, Oxford, U.K., pp. 187-188.
Leung, D. Y. M. et al., "A New In Vitro System for Studying Cell Response to Mechanical Stimulations," Experimental Cell Research, 109 (1977) 285-298.
Banes, A. J. et al., "A New Vacuum-Operated Stress-Providing Instrument That Applies Static or Variable Duration Cyclic Tension or Compression to Cells In Vitro," J. Cell Sci., 1985.
Somjen, D. et al., "Bone Remodelling Induced by Physical Stress in Prostaglandin E.sub.2 Mediated," Biochimica et Biophysica Acta, 627 (1980) 91-100.
Brunette, D. M. et al. "Mechanical Stretching Increases the Number of Epithelial Cells Synthesizing DNA in Culture," J. Cell Sci, 69 (1984) 35-45.
Hasagawa et al., "Mechanical Stretching Increases the Number of Cultured Bone Cells Synthesizing DNA and Alters Their Pattern of Protein Synthesis," Calcif Tissue Int. 37 (1985) 431-436.
Leung, D. Y. M. et al., "Cyclic Stretching Stimulates Synthesis of Matrix Components by Arterial Smooth Muscle Cells In Vitro," Science, 191 (1976) 475-477.

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