Flea membrane binding site proteins as screening tools

Details Flea membrane binding site proteins as screening tools Flea membrane binding site proteins as screening tools

1993-11-12

1995-05-23

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 71, 435820, 530858, C12Q 100, G01N 3353

Patent

active

054181372

ABSTRACT:
The cellular binding proteins present in membranes of fleas, especially those which are present in the digestive tract, are useful as screening tools for systemic anti-flea reagents and in the design of vaccine formulations. Particularly useful is the .alpha.-subunit of (Na.sup.+ /K.sup.+)ATPase. The .alpha.-subunit of this protein expressed in recombinant host cells in the presence of the .beta.-subunit is distributed on the membrane and the recombinant cells can thus be used to screen candidates for ability to bind the cells. Secondary screens are used to determine the specificity of the candidate reagent for flea protein as compared to a corresponding protein derived from other sources.

REFERENCES:
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Strosberg, A. D., et al., Current Opinion in Biotechnology (1991) 2:30-36.

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