Flea epoxide hydrolase nucleic acid molecules, proteins and uses

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4352523, 4353201, 536 232, 536 235, 536 243, 536 2431, C12N 914, C12N 120, C12N 1500, C07H 2104

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active

060371609

ABSTRACT:
The present invention relates to arthropod epoxide hydrolase proteins; to arthropod epoxide hydrolase nucleic acid molecules, including those that encode such epoxide hydrolase proteins; to antibodies raised against such epoxide hydrolase proteins; and to other compounds that inhibit arthropod epoxide hydrolase activity. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising such proteins, nucleic acid molecules, antibodies and/or inhibitory compounds as well as the use of such therapeutic compositions to protect animals from hematophagous arthropod infestation.

REFERENCES:
Meinkoth et al. (1984) Analytical Biochemistry, vol. 138, pp. 267-284.
Khlebodarova et al., 1996, "A Comparative Analysis of Juvenile Hormone Metabolyzing Enzymes in Two Species of Drosophila During Development," Insect Biochem. Molec. Biol., vol. 26:8-9, pp. 829-835.
Roe et al., 1996, "Mechanism of Action and Cloning of Epoxide Hydrolase From the Cabbage Looper, Trichoplusia ni," Archives of Insect Biochemistry and Physiology, vol. 32, pp. 527-535.
Touhara et al., 1993, "Juvenile Hormone Epoxide Hydrolase," The Journal of Biological Chemistry, vol. 268:26, pp. 19604-19609.
Wojtasek et al., 1996, "An Insect Juvenile Hormone-Specific Epoxide Hydrolase Is Related to Vertebrate Microsomal Epoxide Hydrolases," Biochemical and Biophysical Research Communications vol. 220, pp. 323-329.
Skoda et al. (1988) J. Biol. Chem., vol. 263, pp. 1549-1554.

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