Flax ( Linum usitatissimum L.) fatty acid desaturase

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...

Reexamination Certificate

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C435S069100, C435S183000, C435S320100, C435S419000, C435S468000, C435S469000, C536S023100, C536S023200, C536S023600, C800S278000, C800S281000, C800S287000, C800S298000, C530S350000, C530S370000, C530S377000

Reexamination Certificate

active

10165289

ABSTRACT:
The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.

REFERENCES:
patent: 408173167 (1996-07-01), None
patent: WO 94/18337 (1994-08-01), None
patent: WO 98/18948 (1998-05-01), None
patent: WO 01/16340 (2001-03-01), None
Truksa M.; MacKenzie S.L.; Qiu X., Molecular analysis of flax 2S storage protein conlinin and seed specific activity of its promoter Plant Physiology and Biochemistry, vol. 41, No. 2, Feb. 1, 2003, pp. 141-147(7).
Akagi et al (GenEmbl Database, Acces. No. E11610, see result 15, p. 6; Akagi et al, JP408173167A, Jul. 9, 1996).
Xiao Qiu, Haiping Hong, Nagamani Datla, Samuel L. MacKenzie, David C. Taylor, and Terry L. Expression of borage D6 desaturase inSaccharomyces cerevisiaeand oilseed crops Can. J. Bot. 80(1): 42-49 (2002).
Alonso, D.L., et al. “Plants as ‘chemical factories’ for the production of polyunsaturated fatty acids.”Biotechnol Adv.Oct. 2000;18(6):481-97.
Database EMBL Sequence Library Online No. AF047039. “Perilla frutescensomega-3 fatty acid desaturase (FAD3) mRNA, Complete CD's.” Mar. 11, 1999, 2 pages.
Database TREMBL Sequence Library Online No. Q9ZPP7. “Omega-3 fatty acid desaturase,” May 1, 1999, 1 page.
Database EMBL Sequence Library Online No. AF020204. “Pelargonium × hortorumomega-3 desaturase (pxh-15)mRNA.” May 18, 1998, 2 pages.
Database TREMBL Sequence Library Online No. 064907. “Omega-3 desaturase (fragment).” Aug. 1, 1998, 1 page.
Database EMBL Nucleotide Sequence No. X70962. “L usitatissimummRNA for stearoyl-(acyl-carrier-protein) -desaturase.” 3 pages.
Singh, S., et al. “Sequence of a cDNA fromLinum usitatissimumencoding the stearoyl-acyl carrier protein desaturase.”Plant Physiol.Mar. 1994;104(3):1075.
Truksa, M., et al. “Molecular analysis of flax 2S storage protein conlinin and seed specific activity of its promoter.”Plant Physiology and Biochemistry2003 41:141-147.

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