Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;...
Reexamination Certificate
2007-05-01
2010-11-02
McCormick Ewoldt, Susan B (Department: 1661)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
C435S420000, C435S430000, C435S431000, C424S773000
Reexamination Certificate
active
07824914
ABSTRACT:
The present invention provides an in vitro flavonoid-rich rhizome tissue ofNeomarica gracilis, which is obtained from a tissue culture preparation of anN. gracilistissue capable of proliferating, such as a root, a leaf, a basal portion of a leaf, and/or a rhizome. The in vitro flavonoid-rich rhizome tissue ofN. graciliscontains tectorigenin, which is distinctively different from the naturally grown rhizome ofN. graciliswhich contains no tectorigenin. The present invention further provides a method for cultivating the in vitro flavonoid-rich rhizome tissue, a method for extracting the tectorigenin from the flavonoid-rich rhizome tissue, and quantitative methods for determining the amount of tectorigenin in the in vitro flavonoid-rich rhizome tissue.
REFERENCES:
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Tin-Hui Chu; The Rhizome Tissue Multiplacation, Tectorigenin and Total Flavonoid . . . ; Thesis for Master of Science Department of Bioengineering Tatung University; Jul. 2006.
Abdel Nasser B. Singab; Flavonoids fromIris spuria(Zeal) Cultivated in Egypt; Arch Pharm Res; 2004; vol. 27, No. 10; pp. 1023-1028.
Min-Jian Qin; A New Isoflavonoid fromBelamcanda chinensis(L.) DC.; Journal of Integrative Plant Biology; 2005; vol. 47; pp. 1404-1408.
Chu Tin-Hui
Ho Chin-Wen
Andrews & Kurth LLP
McCormick Ewoldt Susan B
Tatung Company
Tatung University
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