Chemistry: molecular biology and microbiology – Vector – per se
Reexamination Certificate
2005-05-17
2005-05-17
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Vector, per se
C435S235100, C435S069100, C536S023100, C536S023720, C536S024100
Reexamination Certificate
active
06893866
ABSTRACT:
The present invention provides a gene expression system comprising: a) a self-replicating expression vector of flavivirus origin which includes the flavivirus 5′ untranslated region (UTR), at least a portion of the 5′ coding region for flavivirus core protein, the nucleotide sequence coding for the flavivirus non-structural proteins, and the complete or most of the 3′-terminal sequence of the flavivrus 3′UTR, required for self-replication of flavivirus genomic material, which vector is adapted to receive at least a nucleotide sequence without disrupting its replication capabilities; and b) at least a second vector that is capable of expressing flavivirus structural protein(s) and any other proteins required for packaging of the self-replicating expression vector into flavivirus viral particles which vector is engineered to prevent recombination with the self-replicating vector when in its presence.
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Khromykh Alexander A.
Varnavski Andrei
Westaway Edwin G.
Fredrikson & Byron , P.A.
Guzo David
The Crown in the Right of the Queensland Department of Health
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